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What would be a hypothesis for the experiment?

What would be a hypothesis for the experiment?

When conducting scientific experiments, researchers develop hypotheses to guide experimental design. A hypothesis is a suggested explanation that is both testable and falsifiable. You must be able to test your hypothesis, and it must be possible to prove your hypothesis true or false.

How do you write a hypothesis using the scientific method?

The scientific method has five basic steps, plus one feedback step:

  1. Make an observation.
  2. Ask a question.
  3. Form a hypothesis, or testable explanation.
  4. Make a prediction based on the hypothesis.
  5. Test the prediction.
  6. Iterate: use the results to make new hypotheses or predictions.

How do you design an experiment and test a hypothesis?

Experimental design means creating a set of procedures to test a hypothesis….

  1. Step 1: Define your research question and variables. You should begin with a specific research question in mind.
  2. Step 2: Write your hypothesis.
  3. Step 3: Design your experimental treatments.
  4. Step 4: Assign your subjects to treatment groups.

What is a positive control for PCR?

The positive control, a known sample of parasite DNA, shows that the primers have attached to the DNA strand. The negative control, a sample without DNA, shows if contamination of the PCR experiment with foreign DNA has occurred.

What are two potential sources of error in the PCR procedure?

The two sources of errors which occur during PCR amplification of DNA are (1) mistakes made by the polymerase and (2) thermal damage of the DNA in double-and single-stranded form.

What will happen if you use too much DNA template in a PCR reaction?

Too much template can shut down Taq. In addition, and as Gary says above, you may also be adding more inhibitors as well – along with too much gDNA.

How can I improve my PCR results?

GC-rich PCR products are difficult to amplify. To improve amplification, increase the annealing temperature. For greater accuracy, optimize the annealing temperature by using a thermal gradient. DMSO or another secondary structure destabilizer can be added (do not exceed 10%).