Press "Enter" to skip to content

What does biuret reagent react with?

The normal color of biuret reagent is blue. The reagent turns violet in the presence of peptide bonds — the chemical bonds that hold amino acids together. The proteins detected must have at least three amino acids, which means that the protein must have at least two peptide bonds.

Why is the biuret test performed under alkaline conditions?

The biuret test measures peptide bonds in a sample. In an alkaline solution, copper II is able to form a complex with the peptide bonds. Once this complex has been formed, the solution turns from a blue color to a purple color. The deeper the purple color, the more peptide-copper complexes that have been formed.

Are all amino acids reactive with the biuret reagent?

Single amino acids and dipeptides do not give the biuret reaction, but tripeptides and larger polypeptides or proteins will react to produce a light blue to violet complex that absorbs light at 540 nm. The intensity of the color produced is proportional to the number of peptide bonds participating in the reaction.

What type of chemical bond causes the biuret reagent to react and give a positive result?

peptide bonds

What is the purpose of the high pH of the biuret reagent?

At higher pH, proteins bind copper forming especially the biuret complex. Proteins mobilize copper ions depending also on pH values. There is a strong relationship between copper ions, proteins and pH that could be of therapeutic importance. Serum copper/iron ratio may be altered also by the changes in pH.

Is biuret solution harmful?

Not a dangerous substance or mixture according to the Globally Harmonised System (GHS). Inhalation May be harmful if inhaled. Causes respiratory tract irritation. Skin May be harmful if absorbed through skin.

How do you dispose of biuret solution?

Contain spill with sand or absorbent material and place in sealed bag or container for disposal. Ventilate and wash spill area after pickup is complete. See Section 13 for disposal information. Handling: Use with adequate ventilation and do not breathe dust or vapor.

What does biuret mean?

Biuret(noun) a white, crystalline, nitrogenous substance, C2O2N3H5, formed by heating urea. It is intermediate between urea and cyanuric acid.

Why should you be careful of biuret solution?

Danger! Corrosive. Causes eye and skin burns. Causes severe digestive and respiratory tract burns.