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How does a buffer keep a solution from becoming too acidic?

How does a buffer keep a solution from becoming too acidic?

Buffers are solutions that contain a weak acid and its a conjugate base; as such, they can absorb excess H+ions or OH– ions, thereby maintaining an overall steady pH in the solution. pH is equal to the negative logarithm of the concentration of H+ ions in solution: pH = – log[H+].

Which of the following accurately describes a buffer?

A buffer is a substance which helps in maintaining a constant pH. Constant pH does not mean that it will change the pH to a neutral value. In fact, it means that it will help to maintain a specific value of pH without changing it.

Which of the following is the best description of how a buffer works?

The statement that best describes a buffer is: A buffer resists change in pH by accepting hydrogen ions when acids are added to the solution and donating hydrogen ions when bases are added.

What is in DNA wash buffer?

Typically, the lysis/binding buffer contains a chaotropic salt (e.g., guanidinium isothiocyanate) whereas the wash buffer contains a high concentration of ethanol (or isopropanol).

What is HBC buffer?

HBC buffer. removes protein from DNA. DNA wash buffer- DNA extraction. remove RNA from DNA.

How can we prevent protein contamination in DNA extraction?

5 Ways to Clean Up A DNA Sample

  1. Phenol-Chloroform Extraction. Phenol chloroform extraction (see Kirby, 1957), normally followed by ethanol precipitation, is the traditional method to remove protein from a DNA sample.
  2. Ethanol Precipitation.
  3. Silica Column-Based Kits.
  4. Anion Exchange.
  5. Magnetic Beads.

What is the function of phenol in DNA extraction?

To obtain nucleic acid samples, the cell must be lysed and the nucleic acids separated from all other cell materials. Phenol is a useful compound for breaking down superfluous cell materials that would otherwise contaminate the nucleic acid sample.

What is the purpose of the warm water bath in DNA extraction?

The hot water bath softens the cell walls and membranes, so the DNA is released. It also further denatures (deactivates) the enzymes in the mixture that can degrade DNA. More is not better, longer heating can denature the DNA.