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How do you observe a specimen under a microscope?

How do you observe a specimen under a microscope?

How to use a microscope

  1. Move the stage (the flat ledge the slide sits on) down to its lowest position.
  2. Place the glass slide onto the stage.
  3. Select the lowest power objective lens.
  4. Turn the coarse focus knob slowly until you are able to see the cells.

What could you determine about a specimen if you observed a slide image showing the specimen with a cell wall but no nucleus or mitochondria?

What could you determine about a specimen if you observed a slide image showing the specimen with a cell wall, but no nucleus or mitochondria? This would describe a prokaryotic cell due to the criteria of having a cell wall, but no nucleus or mitochondria.

What type of microscope is used to observe cells?

light microscopes

What is a specimen in a microscope?

Specimen or slide: The specimen is the object being examined. Most specimens are mounted on slides, flat rectangles of thin glass. The specimen is placed on the glass and a cover slip is placed over the specimen. This allows the slide to be easily inserted or removed from the microscope.

How do you prepare a specimen for a light microscope?

There are two basic types of preparation used to view specimens with a light microscope: wet mounts and fixed specimens. The simplest type of preparation is the wet mount, in which the specimen is placed on the slide in a drop of liquid.১৪ জুন, ২০১৯

Why do cells need to be stained prior to microscopy?

The most basic reason that cells are stained is to enhance visualization of the cell or certain cellular components under a microscope. Cells may also be stained to highlight metabolic processes or to differentiate between live and dead cells in a sample.১৪ জানু, ২০২১

How do you prepare a specimen?

The general requirements for a specimen to be successfully examined using brightfield microscopy are:

  1. That the cells and other elements in the specimen are preserved in a “life-like” state (this process is called “fixation”)
  2. That the specimen is transparent rather than opaque, so that light can pass through it.

Why Iodine is used in plant tissue experiment?

Although onions may not have as much starch as potato and other plants, the stain (iodine) allows for the little starch molecules to be visible under the microscope. Although onions are plants, students will not see any chloroplasts in their slides.

Which form does the stomata cells have?

Essentially, guard cells are two bean-shaped cells that surround a stoma. As epidermal cells, they play an important role in gaseous exchange in and out of plant leaves by regulating the opening and closing of pores known as a stoma.

What is the purpose of using iodine solution?

Iodine is taken by mouth to prevent and treat iodine deficiency and its consequences, including goiter and some thyroid disorders. It is also used for treating lumpy breasts (fibrocystic breast disease) and breast pain (mastalgia).

What is the purpose of lugol’s iodine in Gram staining?

Lugol’s iodine solution is used for Gram staining and can be used as an antiseptic and disinfectant for emergency disinfection of drinking water, and as a reagent for starch detection in routine laboratory and other medical tests.১০ ডিসেম্বর, ২০১০

How can you do Leishman’s staining method?

1) Freshly prepare and rapidly air dry blood film. 2) Cover the film with Leishman’s Stain (S018S) and allow to act for 1 minute. Methanol in the stain fixes the preparation. 3) Add double the volume of distilled water to the slide and mix.

Which stain is used for DLC?

Generally, methylene blue or touline are basic stains, and Eosin, Azure-I, Azure-II are the acidic stains in use. It is advisable to stain a slide soon after preparation of blood smear.

How will you prepare Leishman stains?

Combine 30mL of Leishman’s solution with 150 ml of distilled or demineralized water and with 20 ml of pH 6.8 buffer solution. Slowly add 30 ml of pH 6.8 buffer solution together with 220 ml of destilled or demineralized water into 50 ml of Leishman’s solution. Mix and leave for 10 min.

What is the principle of Leishman stain?

Leishman stain contain methylene blue dye, a basic dye, which gives colour to an acidic component and eosin dye, an acidic dye, which gives colour to a basic component. These dyes differentiate the different component of blood. Leishman’s Stain belongs to the group of Romanowsky stains.

What are the components of Leishman stain?

It consists of a mixture of eosin (an acidic stain), and methylene blue (a basic stain) in alcohol and is usually diluted and buffered before use. It stains the different components of blood in a range of shades between red and blue. The similar Wright’s stain is favoured by American workers.

How blood smear is prepared?

  1. Place clean glass slide on a flat surface. Add one small drop of blood to one end.
  2. Take another clean slide, and holding at an angle of about 45 deg, touch the blood with one end of the slide so the blood runs along the edge of the slide by capillary action.
  3. Make 2 smears, allow to air dry, and label clearly.

What is the purpose of blood smear?

A blood smear is often used as a follow-up test to abnormal results on a complete blood count (CBC) to evaluate the different types of blood cells. It may be used to help diagnose and/or monitor numerous conditions that affect blood cell populations.২৭ জুলাই, ২০১৮

Why do we fix thin smear before staining?

Fix smears of films to minimise cell damage and the production of artefacts. The chemicals used as a fixative can be dangerous and wearing correct PPE, especially gloves, is essential.

What stain is used in preparing a blood smear?

Routine analysis of blood in medical laboratories is usually performed on blood films stained with Romanowsky stains such as Wright’s stain, Giemsa stain, or Diff-Quik. Wright-Giemsa combination stain is also a popular choice.

Whats the advantage of a blood smear compared to a CBC?

Unlike automated tests (such as a CBC), a technician or physician looks at a blood smear under the microscope in order to detect a wide range of changes that give clues to underlying diseases.

What are the characteristics of a good blood smear?

The blood smear must not be too thin or too thick and the tail of the smear must be smooth. The perfect quality smear is influ- enced by three factors: speed, angle and drop size. thinner the smear will be. The slower the slide is moved, the shorter and thicker the slide will be.

What diseases require a peripheral blood smear and why?

Common clinical indications for peripheral blood film analysis include unexplained cytopenia: anaemia, leucopenia or thrombocytopenia; unexplained leukocytosis, lymphocytosis or monocytosis; unexplained jaundice or haemolysis; features of congenital haemolytic anaemias such as splenomegaly, jaundice or bone pains; …